4WVY
Double-heterohexameric rings of full-length Rvb1(ATP)/Rvb2(apo)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-23 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.91889 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 210.348, 210.348, 137.140 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.690 - 3.640 |
| R-factor | 0.2229 |
| Rwork | 0.221 |
| R-free | 0.25060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c9o |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.000 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.21) |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 109.748 | 33.694 | 3.840 |
| High resolution limit [Å] | 3.640 | 11.510 | 3.640 |
| Rmerge | 0.073 | ||
| Rmeas | 0.133 | ||
| Rpim | 0.035 | 0.021 | 0.287 |
| Total number of observations | 190173 | 5701 | 26988 |
| Number of reflections | 13158 | ||
| <I/σ(I)> | 15.9 | 32.7 | 3.9 |
| Completeness [%] | 99.9 | 96 | 100 |
| Redundancy | 14.5 | 13.3 | 14.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 1 M sodium malonate pH 6.0 |
