4WVX
Crystal structure of a phosphotriesterase-like lactonase Gkap in native form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97916 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.398, 89.380, 89.915 |
| Unit cell angles | 90.00, 99.33, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.16757 |
| Rwork | 0.166 |
| R-free | 0.19200 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.474 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 63053 |
| <I/σ(I)> | 16.9 |
| Completeness [%] | 99.8 |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 287 | 14% PEG 20000, 0.1M MES pH6.5 |
