4WVT
Crystal structure of XIAP-BIR2 domain complexed with ligand bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-03 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.200, 38.530, 56.770 |
| Unit cell angles | 90.00, 89.83, 90.00 |
Refinement procedure
| Resolution | 25.150 - 1.960 |
| R-factor | 0.1768 |
| Rwork | 0.174 |
| R-free | 0.23030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.970 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.110 | 0.311 |
| Number of reflections | 10044 | |
| <I/σ(I)> | 12.1 | 3.7 |
| Completeness [%] | 97.2 | 75.3 |
| Redundancy | 3.1 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | .98 M Ammonium Sulfate and .1M Ammonium Formate. Single crystals formed after streak seeding. |
