4WUU

Structure of ESK1 in complex with HLA-A*0201/WT1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979500
Spacegroup name	P 21 21 21
Unit cell lengths	70.080, 118.260, 126.302
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.440 - 3.047
R-factor	0.204
Rwork	0.198
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.742
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	33.440
High resolution limit [Å]	3.047
Rmerge	0.175
Number of reflections	21106
<I/σ(I)>	12.8
Completeness [%]	97.6
Redundancy	12.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.25	291	0.1 M Bis-Tris HCl pH 5.5, 25 percent PEG 3350