4WUJ

Structural Biochemistry of a Fungal LOV Domain Photoreceptor Reveals an Evolutionarily Conserved Pathway Integrating Blue-Light and Oxidative Stress

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-25
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9179
Spacegroup name	P 1 21 1
Unit cell lengths	44.342, 102.225, 71.550
Unit cell angles	90.00, 91.07, 90.00

Refinement procedure

Resolution	44.334 - 2.230
R-factor	0.2087
Rwork	0.206
R-free	0.26020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3d72
RMSD bond length	0.014
RMSD bond angle	1.485
Phasing software	HKL-3000
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.270
High resolution limit [Å]	2.230	6.050	2.230
Rmerge	0.047	0.044	0.118
Total number of observations	121511
Number of reflections	30153
<I/σ(I)>	25.6
Completeness [%]	96.1	84.3	78.3
Redundancy	4	3.8	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	100 mM Hepes pH 7.5 buffer, 6% (v/v) Peg400 and 1.4M ammonium sulfate