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4WUD

N-terminal 43 kDa fragment of the E. coli DNA gyrase B subunit grown from no salt condition

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsDIAMOND BEAMLINE I04-1
Synchrotron siteDiamond
BeamlineI04-1
Temperature [K]100
Detector technologyPIXEL
Collection date2011-09-21
DetectorDECTRIS PILATUS 2M
Wavelength(s)0.917
Spacegroup nameC 2 2 21
Unit cell lengths87.800, 141.480, 80.300
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution27.540 - 1.950
R-factor0.1864
Rwork0.185
R-free0.21910
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1ei1
RMSD bond length0.010
RMSD bond angle1.343
Data reduction softwareXDS
Data scaling softwareAimless
Phasing softwareMOLREP
Refinement softwareREFMAC (5.8.0073)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]27.54027.5402.000
High resolution limit [Å]1.9508.7201.950
Rmerge0.0580.0280.833
Rpim0.0260.0130.372
Total number of observations217252253215998
Number of reflections36686
<I/σ(I)>15.9402.3
Completeness [%]99.797.399.7
Redundancy5.95.56
CC(1/2)0.9990.9990.650
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP829115-25% PEG 3350, 2 mM MgCl2, 100 mM Tris-HCl pH 8.0

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