4WU4

Crystal structure of E. faecalis DNA binding domain LiaRD191N complexed with 22bp DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-01
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	38.336, 77.248, 104.987
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.740 - 2.300
R-factor	0.214
Rwork	0.208
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.602
Data scaling software	HKL-2000
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.097	0.904
<I/σ(I)>	26	2.667
Completeness [%]	99.9	99.2
Redundancy	13.7	11.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.6	283	0.03 M Bis-Tris propane /3.4, 0.07 M Citric acid, 18% PEG 3,350, 0.05% LDAO, 0.1 M Sodium malonate.