4WU1
Complex of 70S ribosome with tRNA-Tyr and mRNA with G-U mismatch in the second position in the P-site
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 210.000, 451.500, 616.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 198.790 - 3.200 |
| Rwork | 0.188 |
| R-free | 0.23820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3TVF 3TVE |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 300.000 | 3.300 | |
| High resolution limit [Å] | 3.200 | 100.000 | 3.200 |
| Rmerge | 0.369 | 0.048 | 3.775 |
| Rmeas | 0.371 | 0.051 | 3.862 |
| Total number of observations | 72141743 | ||
| Number of reflections | 953301 | 27 | 83588 |
| <I/σ(I)> | 14.09 | 17.49 | 1.11 |
| Completeness [%] | 100.0 | 58.7 | 100 |
| Redundancy | 75.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | Tris-acetate, KSCN, PEG 550MME, PEG 20K |
