4WTQ
Structure of the Ssl1 laccase mutant M295L
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-04-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.900002 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.528, 104.795, 164.389 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.580 - 1.800 |
R-factor | 0.17277 |
Rwork | 0.171 |
R-free | 0.20812 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4m3h |
RMSD bond length | 0.019 |
RMSD bond angle | 1.859 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.960 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.070 | 0.508 |
Number of reflections | 82580 | |
<I/σ(I)> | 15.3 | 4 |
Completeness [%] | 99.1 | 99.3 |
Redundancy | 6.3 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 50 mM HEPES, pH 7.0, 1.0-1.3 M ammonium sulfate, 5-20 mM hexamminecobalt(III) chloride |