4WTP
Crystal structure of glycoside hydrolase family 17 beta-1,3-glucanosyltransferase from Rhizomucor miehei
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-24 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.887, 50.366, 98.405 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.490 - 1.300 |
R-factor | 0.17849 |
Rwork | 0.178 |
R-free | 0.18640 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.385 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 27.927 |
High resolution limit [Å] | 1.299 |
Number of reflections | 52655 |
<I/σ(I)> | 71.5 |
Completeness [%] | 92.4 |
Redundancy | 8.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 25% (w/v) PEG 8000, 0.2M (NH4)2SO4 |