4WT1
Complex of 70S ribosome with tRNA-Phe and mRNA with A-A mismatch in the second position in the A-site
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 209.900, 447.900, 621.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 188.030 - 3.050 |
| R-factor | 0.2386 |
| Rwork | 0.199 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3TVE 3TVF |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 250.000 | 3.150 | |
| High resolution limit [Å] | 3.050 | 150.000 | 3.050 |
| Rmerge | 0.278 | 0.148 | 5.486 |
| Rmeas | 0.162 | 5.527 | |
| Total number of observations | 170925258 | ||
| Number of reflections | 1097157 | 3 | 100127 |
| <I/σ(I)> | 27.9 | 16.45 | 1.08 |
| Completeness [%] | 99.8 | 20 | 99.4 |
| Redundancy | 155.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | Tris-acetate, KSCN, PEG 550MME, PEG 20K |
