4WSZ
Crystal structure of the DNA binding domains of wild type LiaR from E. faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 31.232, 77.024, 77.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.952 - 1.769 |
| R-factor | 0.172 |
| Rwork | 0.164 |
| R-free | 0.20810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.908 |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.810 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.067 | 0.335 |
| <I/σ(I)> | 7.244 | |
| Completeness [%] | 97.7 | 94 |
| Redundancy | 11.6 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 283 | 0.2 M LiSO4, 25%PEG 3.350, 0.05%Tween-20,10%Glycerol, 10 mM Praseodymium (III) acetate hydrate |
