4WRM
Structure of the human CSF-1:CSF-1R complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-07-05 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0015 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 281.470, 281.470, 91.170 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.752 - 6.853 |
| R-factor | 0.3299 |
| Rwork | 0.326 |
| R-free | 0.35930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4WRL and 4LIQ |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1685)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.752 | 7.210 |
| High resolution limit [Å] | 6.800 | 6.800 |
| Rmerge | 0.071 | 1.020 |
| Number of reflections | 3953 | |
| <I/σ(I)> | 21.33 | 2.98 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 10.2 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1 M NaCl, 0.1 M Tris pH 8.0 and 8% w/v PEG 20000 |
