4WR3
Y274F alanine racemase from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-10-17 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 6 |
| Unit cell lengths | 148.079, 148.079, 163.689 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.720 - 1.900 |
| R-factor | 0.17686 |
| Rwork | 0.175 |
| R-free | 0.20371 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rjg |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.349 |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.720 | 1.900 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Number of reflections | 168143 | |
| <I/σ(I)> | 18.5 | 1.9 |
| Completeness [%] | 99.6 | 93.8 |
| Redundancy | 22.1 | 16.4 |
| CC(1/2) | 0.998 | 0.632 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.1 M HEPES pH 7.0, 1.6 M ammonium sulfate |
