4WQ5
YgjD(V85E)-YeaZ heterodimer in complex with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2012-12-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 1 |
| Unit cell lengths | 63.690, 68.080, 87.340 |
| Unit cell angles | 109.31, 92.56, 117.94 |
Refinement procedure
| Resolution | 42.620 - 2.330 |
| R-factor | 0.1825 |
| Rwork | 0.180 |
| R-free | 0.23323 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.313 |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.330 |
| Number of reflections | 48866 |
| <I/σ(I)> | 13.06 |
| Completeness [%] | 96.0 |
| Redundancy | 3.34 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M HEPES pH 7.5, 26% PEG4000, 0.3 M Lithium Sulfate and 0.1 Sodium Acetate |
