4WQ1

Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-03-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	209.900, 448.700, 618.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	151.800 - 3.100
Rwork	0.188
R-free	0.23460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3TVF 3TVE
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	300.000		3.200
High resolution limit [Å]	3.100	100.000	3.100
Rmerge	0.405	0.042	3.859
Rmeas	0.407	0.049	3.919
Total number of observations	83223436
Number of reflections	1043636	12	94258
<I/σ(I)>	16.08	23.5	1.32
Completeness [%]	99.9	26.1	99.9
Redundancy	79.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	Tris-acetate, KSCN, PEG 550MME, PEG 20K