4WQ1
Complex of 70S ribosome with tRNA-Tyr and mRNA with C-A mismatch in the first position in the A-site.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 209.900, 448.700, 618.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 151.800 - 3.100 |
| Rwork | 0.188 |
| R-free | 0.23460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3TVF 3TVE |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 300.000 | 3.200 | |
| High resolution limit [Å] | 3.100 | 100.000 | 3.100 |
| Rmerge | 0.405 | 0.042 | 3.859 |
| Rmeas | 0.407 | 0.049 | 3.919 |
| Total number of observations | 83223436 | ||
| Number of reflections | 1043636 | 12 | 94258 |
| <I/σ(I)> | 16.08 | 23.5 | 1.32 |
| Completeness [%] | 99.9 | 26.1 | 99.9 |
| Redundancy | 79.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | Tris-acetate, KSCN, PEG 550MME, PEG 20K |
