4WPB
Vascular endothelial growth factor in complex with alpha/beta-VEGF-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.100, 78.400, 56.500 |
| Unit cell angles | 90.00, 102.80, 90.00 |
Refinement procedure
| Resolution | 45.078 - 3.110 |
| R-factor | 0.2454 |
| Rwork | 0.240 |
| R-free | 0.29830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.475 |
| Data reduction software | XDS (November 11, 2013) |
| Data scaling software | XSCALE (January 10, 2014) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (1.9-1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.080 | 3.180 | |
| High resolution limit [Å] | 3.100 | 13.870 | 3.100 |
| Rmerge | 0.083 | 0.037 | 0.243 |
| Rmeas | 0.097 | 0.045 | 0.283 |
| Total number of observations | 22316 | ||
| Number of reflections | 6096 | 70 | 447 |
| <I/σ(I)> | 14.26 | 20.54 | 7.45 |
| Completeness [%] | 99.6 | 95.9 | 100 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M magnesium formate dihydrate, 15% (w/v) PEG3350, 30% (v/v) glycerol |
