4WOY

Crystal structure and functional analysis of MiD49, a receptor for the mitochondrial fission protein Drp1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-23
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.99
Spacegroup name	C 2 2 21
Unit cell lengths	100.674, 153.390, 109.015
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.980 - 2.400
R-factor	0.2067
Rwork	0.206
R-free	0.24290
RMSD bond length	0.009
RMSD bond angle	1.355
Refinement software	PHENIX ((phenix.refine: dev_1760))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.980	2.480
High resolution limit [Å]	2.400	2.400
Rmerge	0.051	0.694
Number of reflections	32951
<I/σ(I)>	21.5	2.1
Completeness [%]	98.9	99.51
Redundancy	4.3	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	298	100 mM HEPES (pH 7.0), 20 mM MgCl2, 20% polyacrylic acid 5,100 (w/v)