4WO7

Crystal Structure of PrsA from Bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-13
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.36246
Spacegroup name	C 2 2 21
Unit cell lengths	69.290, 87.680, 234.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.364 - 2.630
R-factor	0.2275
Rwork	0.226
R-free	0.25120
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.040
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: dev_1779))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	117.000	2.790
High resolution limit [Å]	2.630	2.630
Rmerge	0.054
Number of reflections	21428
<I/σ(I)>	19.7	1.6
Completeness [%]	98.8	90.2
Redundancy	6.7	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	303.15	17 % PEG2000MME, 0.1M Hepes pH 7.5