4WO4

The molecular bases of Delta/Alpha beta T cell-mediated antigen recognition.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.954
Spacegroup name	P 21 21 21
Unit cell lengths	78.930, 79.250, 189.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.600 - 2.500
R-factor	0.1963
Rwork	0.194
R-free	0.23680
RMSD bond length	0.010
RMSD bond angle	1.140
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.600	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	41804
<I/σ(I)>		2.5
Completeness [%]		100
Redundancy		4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.1	293	27% PEG1500 0.1M Sodium Malonate-imidazole-borate (2:3:3 molar ratio) 3% PEG3350