4WO2
CRYSTAL STRUCTURE OF HUMAN NATIVE CKIT PROTO-ONCOGENE PROMOTER QUADRUPLEX DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9173 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 31.440, 31.440, 186.810 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.290 - 1.820 |
| R-factor | 0.211 |
| Rwork | 0.210 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qxr |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.694 |
| Data reduction software | xia2 |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.130 | 1.860 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.083 | |
| Number of reflections | 10436 | |
| <I/σ(I)> | 14.5 | |
| Completeness [%] | 99.1 | |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 283 | MPD, POTASSIUM CHLORIDE, MAGNESIUM CHLORIDE, POTASSIUM CACODYLATE, PH 6.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 283K |
