4WNI

Crystal structure of the T229K mutant of human GAPDH at 2.3 angstroems resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-24
Detector	ADSC QUANTUM 315
Wavelength(s)	1.127
Spacegroup name	P 21 21 21
Unit cell lengths	81.363, 133.095, 147.768
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.500 - 2.300
R-factor	0.169
Rwork	0.169
R-free	0.19800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u8f
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.500	2.380
High resolution limit [Å]	2.300	2.300
Rmerge		0.235
Number of reflections	63669
<I/σ(I)>	10.7	12.1
Completeness [%]	88.5	55.4
Redundancy	4.8	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	20% (w/v) PEG3350, 0.02 M ZnCl2