4WNG

Crystal structure of the TPR domain of LGN in complex with Frmpd4/Preso1 at 2.1 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2014-02-10
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.1000
Spacegroup name	P 61 2 2
Unit cell lengths	90.527, 90.527, 177.067
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.580 - 2.110
R-factor	0.2268
Rwork	0.224
R-free	0.27120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wne
RMSD bond length	0.009
RMSD bond angle	1.066
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP (11.0.05)
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.140
High resolution limit [Å]	2.100	5.700	2.100
Rmerge	0.093	0.040	0.951
Rmeas	0.096	0.041	0.985
Rpim	0.023	0.010	0.257
Total number of observations	442380
Number of reflections	25343
<I/σ(I)>	8.2		2.308
Completeness [%]	99.9	99.2	100
Redundancy	17.5	17.1	14.4
CC(1/2)		0.999	0.915

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1 M BisTrispropane (pH 8.0), 0.2 M magnesium chloride, 20% PEG 3350