4WNF

Crystal structure of the oxidized TPR domain of LGN in complex with Frmpd4/Preso1 at 2.9 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	90
Detector technology	CCD
Collection date	2013-12-12
Detector	RAYONIX MX300HE
Wavelength(s)	0.9000
Spacegroup name	P 61 2 2
Unit cell lengths	92.599, 92.599, 175.238
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.120 - 2.900
R-factor	0.2391
Rwork	0.237
R-free	0.28020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wne
RMSD bond length	0.009
RMSD bond angle	1.138
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP (11.0.05)
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.950
High resolution limit [Å]	2.900	7.860	2.900
Rmerge	0.075	0.022	0.502
Total number of observations	74336
Number of reflections	10385
<I/σ(I)>	15		2.728
Completeness [%]	99.3	91.6	100
Redundancy	7.2	6.1	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M BisTrispropane (pH 7.5), 0.2 M potassium thiocyanate, 20% PEG 3350