4WN9
Structure of the Nitrogenase MoFe Protein from Clostridium pasteurianum Pressurized with Xenon
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.309, 73.446, 108.658 |
| Unit cell angles | 73.47, 87.56, 83.98 |
Refinement procedure
| Resolution | 39.750 - 1.900 |
| R-factor | 0.18215 |
| Rwork | 0.179 |
| R-free | 0.23427 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.346 |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.750 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 137866 |
| <I/σ(I)> | 16 |
| Completeness [%] | 96.7 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 297 | PEG 3350, lithium citrate, sodium dithionite |
