4WMB
crystal structure of mouse Xyloside xylosyltransferase 1 complexed with manganese, acceptor ligand and UDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1000 |
| Spacegroup name | P 3 |
| Unit cell lengths | 89.468, 89.468, 42.934 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 77.480 - 2.050 |
| R-factor | 0.20249 |
| Rwork | 0.201 |
| R-free | 0.23466 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.260 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.053 | 0.429 |
| Number of reflections | 24139 | |
| <I/σ(I)> | 17.1 | 2.5 |
| Completeness [%] | 99.9 | 99 |
| Redundancy | 4.8 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2 M Li2SO4, 0.1 M Bis-Tris, pH 6.5, and 21% PEG3350 |
