4WMA
Crystal structure of mouse Xyloside xylosyltransferase 1 complexed with manganese,acceptor ligand and UDP-Glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1000 |
| Spacegroup name | P 3 |
| Unit cell lengths | 90.028, 90.028, 43.136 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 77.970 - 1.620 |
| R-factor | 0.22034 |
| Rwork | 0.219 |
| R-free | 0.24252 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.370 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.970 | 1.680 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.045 | 0.592 |
| Number of reflections | 49949 | |
| <I/σ(I)> | 28.2 | 2.2 |
| Completeness [%] | 99.9 | 98.8 |
| Redundancy | 9.6 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2 M Li2SO4, 0.1 M Bis-Tris, and 21% PEG3350 |
