4WM8

Crystal Structure of Human Enterovirus D68

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-13
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9787
Spacegroup name	I 2 2 2
Unit cell lengths	327.000, 347.800, 357.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.250 - 2.000
Rwork	0.275
R-free	0.27900
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-3000
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.070
High resolution limit [Å]	2.000	4.310	2.000
Rmerge	0.105	0.061	0.754
Rmeas	0.131	0.074	0.951
Rpim	0.077	0.040	0.571
Total number of observations	1586943
Number of reflections	748623
<I/σ(I)>	9.3
Completeness [%]	55.7	52.5	41.6
Redundancy	2.1	2.5	2
CC(1/2)		0.992	0.654

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M Sodium Acetate (pH 4.5), 3.5 M Sodium Formate