4WLW
CRYSTAL STRUCTURE OF THE AG(I) (ACTIVATOR) FORM OF E. COLI CUER, A COPPER EFFLUX REGULATOR, BOUND TO COPA PROMOTER DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97850 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 162.965, 162.965, 53.175 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.930 - 2.800 |
| R-factor | 0.21203 |
| Rwork | 0.209 |
| R-free | 0.26416 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q06 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.574 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.420 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.071 | 0.350 |
| Number of reflections | 10637 | |
| <I/σ(I)> | 16.4 | 3.6 |
| Completeness [%] | 99.4 | 97.4 |
| Redundancy | 6.6 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 296 | 0.1M IMIDAZOLE, PH 8.0, 0.2M CA(C2H3O2)2 AND 8% PEG8000, VAPOR DIFFUSION, HANGING DROP TEMPERATURE 296K |
