4WLU
Crystal structure of L-malate and NAD bound MDH2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-25 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97934 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.982, 152.140, 155.035 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.790 - 2.140 |
R-factor | 0.21757 |
Rwork | 0.216 |
R-free | 0.24420 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.984 |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.790 |
High resolution limit [Å] | 2.140 |
Number of reflections | 74182 |
<I/σ(I)> | 55.5 |
Completeness [%] | 99.8 |
Redundancy | 13.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 288 | 0.1 M Na2HPO4:citric acid, 40% (v/v) polyethylene glycol 300, 10% DMSO |