4WLP

Crystal structure of UCH37-NFRKB Inhibited Deubiquitylating Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-28
Detector	PSI PILATUS 6M
Wavelength(s)	0.97945
Spacegroup name	P 41 2 2
Unit cell lengths	95.488, 95.488, 131.964
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.680 - 3.102
R-factor	0.1999
Rwork	0.193
R-free	0.26800
RMSD bond length	0.009
RMSD bond angle	1.109
Data reduction software	XDS
Data scaling software	Aimless

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.680	38.680	3.320
High resolution limit [Å]	3.100	8.770	3.100
Rmerge	0.132	0.020	1.511
Rpim	0.038	0.006	0.433
Total number of observations	149789	6511	26555
Number of reflections	11634
<I/σ(I)>	17.6	83	2
Completeness [%]	99.9	98.8	100
Redundancy	12.9	11	12.9
CC(1/2)	0.999	1.000	0.761

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	294	16-18% PEG3350, 100-300 mM Ammonium Citrate, 7.0 vapor diffusion hanging drop, 2:1 or 2:2 ratios protein to reservoir