4WLO

Crystal structure of oxaloacetate and NADH bound MDH2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-28
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97933
Spacegroup name	P 21 21 21
Unit cell lengths	59.989, 152.191, 155.869
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.350 - 2.500
R-factor	0.21373
Rwork	0.212
R-free	0.25488
RMSD bond length	0.006
RMSD bond angle	1.066
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	28.350
High resolution limit [Å]	2.500
Number of reflections	47440
<I/σ(I)>	47.8
Completeness [%]	99.0
Redundancy	12.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	288	0.1 M Na2HPO4:citric acid, 40% (v/v) polyethylene glycol 300, 10% DMSO