4WLE
Crystal structure of citrate bound MDH2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-04-09 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.900 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.695, 151.220, 154.645 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.100 - 1.900 |
| R-factor | 0.2194 |
| Rwork | 0.218 |
| R-free | 0.23806 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dfd |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.898 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 45.100 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 104532 |
| <I/σ(I)> | 31.3 |
| Completeness [%] | 99.8 |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 288 | 0.1M Na2HPO4, citric acid, 40% (v/v) polyethylene glycol 300 |
