4WL1

Structure of WzzE Polysaccharide Co-polymerase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	85
Detector technology	CCD
Collection date	2013-08-06
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	276.849, 246.306, 133.201
Unit cell angles	90.00, 89.99, 90.00

Refinement procedure

Resolution	47.810 - 5.989
R-factor	0.2369
Rwork	0.234
R-free	0.24550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3b8o
RMSD bond length	0.012
RMSD bond angle	2.248
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.810	6.200
High resolution limit [Å]	5.990	5.900
Number of reflections	43381
<I/σ(I)>	11.73	1.15
Completeness [%]	96.4	83.54
Redundancy	3.5	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	298	8% PEG 8000, 0.1M Bis-Tris pH 6.5, 0.2M NaCl, 15% glycerol