4WKZ

Complex of autonomous ScaG cohesin CohG and X-doc domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-16
Detector	ADSC QUANTUM 315
Wavelength(s)	.97250
Spacegroup name	P 21 21 2
Unit cell lengths	74.029, 211.781, 36.666
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	105.890 - 1.790
R-factor	0.18075
Rwork	0.179
R-free	0.22273
RMSD bond length	0.016
RMSD bond angle	1.525
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.820
High resolution limit [Å]	1.790	1.790
Rmerge	0.180	0.333
Number of reflections	56262
<I/σ(I)>	8.1	1.85
Completeness [%]	87.2
Redundancy	4.2	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	No 37 of Crystal Screen kit from Hampton Research