4WK1
Crystal structure of Staphylococcus aureus PstA in complex with c-di-AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-10 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.99998 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 68.100, 68.100, 135.740 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.517 - 1.980 |
| R-factor | 0.192 |
| Rwork | 0.188 |
| R-free | 0.22880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3m05 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.201 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Number of reflections | 8523 | |
| <I/σ(I)> | 23.6 | 2 |
| Completeness [%] | 97.7 | 81.4 |
| Redundancy | 16.6 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 6 % w/v PEG 8000, 0.1 M HEPES pH 7.0, 0.2 M calcium-acetate |
