4WJW
Crystal Structure of the Chs5-Chs6 Exomer Cargo Adaptor Complex Bound to portion of Chs3
Replaces: 4U9TExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-16 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | .9767 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 218.613, 218.613, 137.848 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.650 - 2.590 |
R-factor | 0.181 |
Rwork | 0.180 |
R-free | 0.21100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4GNS |
Refinement software | PHENIX ((PHENIX.REFINE: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.650 | 2.683 |
High resolution limit [Å] | 2.590 | 2.590 |
Number of reflections | 59936 | |
<I/σ(I)> | 19.45 | |
Completeness [%] | 99.0 | 99 |
Redundancy | 12.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 0.3 M AMMONIUM SULFATE, 0.1 M CITRIC ACID |