4WJV
Crystal structure of Rsa4 in complex with the Nsa2 binding peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-09-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.3 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 198.578, 96.493, 196.445 |
| Unit cell angles | 90.00, 115.45, 90.00 |
Refinement procedure
| Resolution | 48.985 - 3.200 |
| R-factor | 0.1946 |
| Rwork | 0.192 |
| R-free | 0.24380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4WJU and 4EDQ |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.696 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.985 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.168 | |
| Number of reflections | 55664 | |
| <I/σ(I)> | 15.6 | 3.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.4 | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2M NH4SO4, 20% PEG 3350 |
