4WJT
Stationary Phase Survival Protein YuiC from B.subtilis complexed with NAG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-19 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | H 3 |
| Unit cell lengths | 145.480, 145.480, 37.820 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 72.740 - 1.210 |
| R-factor | 0.11235 |
| Rwork | 0.111 |
| R-free | 0.14434 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ae0 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.156 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | ACORN |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.370 | 1.230 |
| High resolution limit [Å] | 1.210 | 1.210 |
| Rmerge | 0.043 | 0.569 |
| Number of reflections | 89967 | |
| <I/σ(I)> | 10.3 | 2.4 |
| Completeness [%] | 98.8 | 97.2 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 289 | 5mM NAG, 48% (v/v) polypropylene glycol P400, 0.1M HEPES pH 6, 3% (v/v) DMSO |
