4WJS
Crystal structure of Rsa4 from Chaetomium thermophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-03 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.155, 48.832, 58.625 |
| Unit cell angles | 67.47, 88.39, 62.65 |
Refinement procedure
| Resolution | 42.070 - 1.800 |
| R-factor | 0.1676 |
| Rwork | 0.165 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nex |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.014 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.070 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.044 | 0.284 |
| Number of reflections | 37846 | |
| <I/σ(I)> | 6.7 | 2.5 |
| Completeness [%] | 94.7 | 93.3 |
| Redundancy | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 mM KF, 20% PEG 3350 |
