4WJL

Structure of human dipeptidyl peptidase 10 (DPPY): a modulator of neuronal Kv4 channels

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-08
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97943
Spacegroup name	P 21 21 21
Unit cell lengths	80.910, 143.730, 176.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	66.546 - 3.400
R-factor	0.2092
Rwork	0.207
R-free	0.24190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xfd
RMSD bond length	0.008
RMSD bond angle	1.362
Data reduction software	iMOSFLM
Data scaling software	SCALA (3.3.9)
Phasing software	PHASER (2.1.4)
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	111.388	66.546	3.580
High resolution limit [Å]	3.400	10.750	3.400
Rmerge		0.053	0.775
Rmeas	0.251
Rpim	0.108	0.026	0.378
Total number of observations	149063	4860	20630
Number of reflections	29019
<I/σ(I)>	6.8	18.2	2.3
Completeness [%]	99.9	99.2	99.9
Redundancy	5.1	4.8	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	Crystals formed within 2-4 weeks using 100 micrometers of reservoir solution consisting of 20%(w/v) PEG 1500, 0.2 M MgCl2, 0.1 M sodium cacodylate pH 5.5.