4WJ4

Crystal structure of non-discriminating aspartyl-tRNA synthetase from Pseudomonas aeruginosa complexed with tRNA(Asn) and aspartic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-02
Detector	RAYONIX MX225HE
Wavelength(s)	0.9
Spacegroup name	P 41 2 2
Unit cell lengths	157.913, 157.913, 146.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.478 - 3.294
R-factor	0.1912
Rwork	0.189
R-free	0.22440
RMSD bond length	0.003
RMSD bond angle	0.615
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	47.260
High resolution limit [Å]	3.290
Number of reflections	28534
<I/σ(I)>	14.53
Completeness [%]	99.8
Redundancy	14.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.7	298	Trisodium citrate