Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 125.890, 58.672, 90.147 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.336 - 2.850 |
| R-factor | 0.2294 |
| Rwork | 0.227 |
| R-free | 0.28190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3LFX |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.121 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1702)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.340 | 3.000 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.130 | 0.640 |
| Number of reflections | 15944 | |
| <I/σ(I)> | 9.7 | 3.1 |
| Completeness [%] | 98.8 | |
| Redundancy | 4.7 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 298 | 19% PEG 3350, 0.1M CITRIC ACETATE |
