4WIM
Crystal Structure of the GMP Synthetase from Plasmodium falciparum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97239 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.453, 68.548, 102.080 |
| Unit cell angles | 101.11, 99.17, 93.71 |
Refinement procedure
| Resolution | 49.305 - 3.600 |
| R-factor | 0.2821 |
| Rwork | 0.281 |
| R-free | 0.29270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uow |
| RMSD bond length | 0.035 |
| RMSD bond angle | 3.448 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.600 | 3.960 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.640 | |
| Number of reflections | 13123 | |
| <I/σ(I)> | 7.2 | 1.34 |
| Completeness [%] | 88.8 | 84 |
| Redundancy | 1.7 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 292 | 20% (w/v) PEG 3350, 0.2 M Diammonium tartrate and 5 mM NaCl |
