4WIK
HUMAN SPLICING FACTOR, CONSTRUCT 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 127.399, 180.416, 57.029 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.200 - 3.000 |
| R-factor | 0.244 |
| Rwork | 0.242 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.200 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.083 | 0.694 |
| Number of reflections | 13401 | |
| <I/σ(I)> | 8.7 | 1.9 |
| Completeness [%] | 99.2 | 99.8 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M SODIUM PHOSPHATE DIBASIC DIHYDRATE, 22% (W/V) POLYETHYLENE GLYCOL 3350, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K |
