4WIJ
HUMAN SPLICING FACTOR, CONSTRUCT 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 66.580, 66.580, 398.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.080 - 3.490 |
| R-factor | 0.275 |
| Rwork | 0.272 |
| R-free | 0.33500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.880 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.080 | 3.670 |
| High resolution limit [Å] | 3.480 | 3.480 |
| Rmerge | 0.100 | 0.307 |
| Number of reflections | 12337 | |
| <I/σ(I)> | 10.8 | 4.3 |
| Completeness [%] | 99.7 | 98.1 |
| Redundancy | 6.8 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 26% (V/V) 1,4 DIOXANE, 0.05M MOPS, 50 MM MAGNESIUM CHLORIDE HEXAHYDRATE, 1 MM SPERMINE, PH 7.0, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K |
