4WII

HUMAN SPLICING FACTOR, CONSTRUCT 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-15
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9686
Spacegroup name	P 21 21 21
Unit cell lengths	63.447, 67.611, 119.528
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.520 - 2.050
R-factor	0.201
Rwork	0.199
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.040
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.610	2.160
High resolution limit [Å]	2.050	2.050
Number of reflections	32938
<I/σ(I)>	13.1	2.1
Completeness [%]	99.9	100
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	1.9 M AMMONIUM SULFATE, 0.1 M TRIS, 20% ETHYLENE GLYCOL (V/V), PH 8.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K