4WHU
BROMO domain of CREB binding protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-11-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 25.284, 43.317, 51.387 |
Unit cell angles | 90.00, 98.68, 90.00 |
Refinement procedure
Resolution | 32.960 - 2.110 |
R-factor | 0.2101 |
Rwork | 0.208 |
R-free | 0.26120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dvw |
RMSD bond length | 0.008 |
RMSD bond angle | 0.950 |
Refinement software | BUSTER (2.11.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.960 | 2.130 |
High resolution limit [Å] | 2.110 | 2.100 |
Rmerge | 0.069 | 0.498 |
Number of reflections | 6356 | |
<I/σ(I)> | 24.6 | 4.3 |
Completeness [%] | 99.3 | 90.9 |
Redundancy | 3.9 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | obtained through screen with Hampton Research kits (HT and Index) |