4WHJ

Myxovirus Resistance Protein 2 (MxB)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-09
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	53.185, 80.782, 183.666
Unit cell angles	90.00, 95.73, 90.00

Refinement procedure

Resolution	43.929 - 3.200
R-factor	0.2667
Rwork	0.265
R-free	0.29930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3szr
RMSD bond length	0.002
RMSD bond angle	0.630
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.260
High resolution limit [Å]	3.200	8.670	3.200
Rmerge	0.089	0.040
Total number of observations	132310
Number of reflections	24322
<I/σ(I)>	8.9
Completeness [%]	94.8	97	89.9
Redundancy	5.4	6	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		298	0.1 M MES, 5% PEG4000, 10% 2-Propanol, 0.05 M magnesium chloride