4WHC
Human CEACAM6 N-domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 63 |
Unit cell lengths | 91.912, 91.912, 60.359 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.100 - 2.705 |
R-factor | 0.1922 |
Rwork | 0.188 |
R-free | 0.22660 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.459 |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.100 |
High resolution limit [Å] | 2.700 |
Number of reflections | 8009 |
<I/σ(I)> | 35.9 |
Completeness [%] | 99.4 |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 20% PEG 3000, 100 mM Imidazole pH 8.0, 200 mM ZnAc |